Toxoplasmosis is a parasitic infection with public health importance. When pregnant women acquires infection, it may led to congenital toxoplasmosis culminating in fetal losses and congenital abnormalities. A cross-sectional study was conducted on slaughtered sheep in Mandeeq Slaughter House and Pregnant Women attending antenatal care at in Hargiesa group from June 2021 to December 2021.Therefore, this study was designed to determine the seroprevalence of toxoplasmosis in sheep and pregnant women attending antenatal care at Hargiesa Group Hospital. Four hundred sera were collected from slaughtered sheep at Mandeeq slaughter house and two hundred serum samples were taken from pregnant women attending Hargiesa group hospital. Data on socio-demographic and potential risk factors were collected from each study using systematic random sampling method. Sera were examined for anti-Toxoplasma gondii antibodies using latex agglutination test. Multiple logistic regression model was used to establish the association between toxoplasmosis and associated potential risk factors and P-value less than 0.05 were considered to be statistically significant. The overall prevalence of toxoplasmosis in sheep slaughtered in Mandeeq Slaughter House during the study period were 25.5% and 28% in sheep and human respectively. Multivariable logistic regression analysis indicated that female (AOR = 2.18; 95% CI: 1.38 - 3.47; p = 0.001) and young age groups (AOR = 3.04; 95% CI: 1.04-8.86; p = 0.041) of sheep were significantly associated with Toxoplasma gondii sero-positivity. In pregnant women, age groups between 25-34 AOR = 2. 76; 95% CI: 1.07 - 7.14; P = 0.037), pregnant women who have cats in their home (AOR = 6.45; 95% CI: 2.37-17.52 ; P = 0.000) ,women who have close contact with garden soil (AOR = 6.74; 95% CI: 2.55-17.81 ; P = 0.000), poor hand washing before eating (AOR = 29.5; 95% CI: 5.41– 161.11 ; P= 0.004) and drinking tap water (AOR = 9.99; 95% CI: 2.75 - 36.12; P = 0.013) were significantly associated with Toxoplasma gondii sero-positivity. In conclusion, toxoplasmosis is prevalent in Hargeisa. Therefore, pregnant women should avoid close contact with cats and garden soil.