Campylobacter is one of the most significant foodborne pathogens in the world and poultry is considered as the main reservoir of the bacteria. Although the significance of commercial poultry in the development of campylobacteriosis is well documented, little is known about the possible contribution of backyard chickens as a direct animal/faecal contact or exposure pathway for consumption. A cross-sectional study was conducted from February 2021 to February 2022 to isolate and identify Campylobacter species and assess its associated risk factors from backyard chicken feces in Bahir Dar zuria district. Using standard cultural and biochemical techniques, a total number of 179 samples were subjected to Campylobacter isolation and identification. During sample collection data on backyard chicken management, biosecurity, and hygiene practices were collected using a structured questionnaire. The risk factors were analyzed using univariable and multivariable logistic regression with the significant levels of P-value less than 0.05. Out of 179 fecal samples processed, the rate of recovery of Campylobacters was 71 (39.7%); of which 51 (71.83%) were found to be C. jejuni, 9(12.67%) were C. coli, 4(5.63%) were C. lari and 7(9.86%) were unidentified isolates. Regarding to risk factors the multivariable logistic regression analysis revealed that Campylobacter positivity was significantly increased by the following factors: chickens kept with other animals (OR = 3.26; 95% CI = 1.42- 7.47), backyard spread of manure (OR: 11.29; 95% CI: 2.36 - 54.02) and chickens from rural areas (OR = 9.06; 95% CI = 3.59-22. 84). However, the presence of working latrine had protective effect (OR = 0.25, 95% CI: 0.12 - 0.60). The present study revealed the presence of high level of Campylobacter species in