Floriculture is one the emerging horticultural crops industry in Ethiopia; however there are different pre-harvest and post-harvest production constraints that affect the development of the industry .Pre-cooling time is one of the factors that determine the post-harvest quality and the shelf life of cut flowers. A laboratory experiment was conducted at Tana Flora PLC in 2022 to assess the influence of pre-cooling times on the vase life of different rose varieties. The experiment consisted of five (5) pre-cooling treatments (zero hour, two hours, four hours, six hours and eight hours) and four (4) rose varieties (Orchestra, Bellerose, Furiosa and Athena) arranged in factorial combination with CRD in three replications. Six (6) flowers of each treatment were pre-cooled using different pre-cooling times at 8°C temperature inside precooling room. The main and interaction effects of Pre-cooling times and varieties were very highly significant (P < 0.0001) on water loss, water balance, days to full blooming and dry matter contents. In addition, the main effects of both factors were very highly significant (P<0.0001) on vase life, bud diameter and relative fresh weight change, while their interaction effect were showed significant (P<0.05) differences. Bellerose variety pre-cooled for six hours and four hours performed a better blooming day (9days), vase life (17days), head diameter (7cm), water balance (-4.0ml/stem/day), relative fresh weight change (82.22%) and dry matter contents (22.22%). Cut rose flower variety Bellerose pre-cooled for four hours and six hours were recorded the longest vase life (17days), followed by Athena and Furiosa (15.67days) and Orchestra (15.16 days). On the other hand, Orchestra pre-cooled for zero hour also recorded the shortest vase life (11.33days) during the study. Thus, it is advised to pre-cool the flowers for four up to six hours to maintain the freshness and vase life during post harvest handling. The findings of the study provide an alternative practices to extend the vase life of cut roses and its quality for the satisfaction of flower users and consumers.