Natural pastures are becoming less productive due to high grazing intensity. To meet the demand for green fodder, hydroponic techniques are alternatives to supplement pasture. A study was conducted in the Fogera district to evaluate the effects of harvesting age and barley varieties on morphological characteristics, biomass yield, chemical composition, and economic benefits under hydroponics. HB-1307, Debark-1, Tila, and Local barley at 6, 8, 10 , and 12 harvestings were used. Data were subjected to a GLM analysis of variance procedures SAS version 9.2. All morphological parameters except a number of leaves per plant (NLPP), stem weight (SW), leaf-to-stem ratio (LSR) & all chemical compositions were significantly different (P<0.01) harvesting age (HA) and different barley varieties. Plant height, shoot length, leaf length, and all chemical compositions were significantly different (P<0.01) by the interaction effects. The highest plant height (21.26 cm) and (21.39%) CP were obtained from Debark-1 on the 12 days of harvesting. The highest fresh fodder biomass yield (FFBY) 203.50 t/ha, and dry matter yield (DMY) 36.21 t/ha were obtained at 12 days of harvesting. In the case of harvesting age, all morphological parameters increased in the progress harvesting age, except for LSR. All morphological characteristics were significantly affected (P<0.001) by different barley varieties except for the NLPP, SW, and LSR. The highest net return 2,923,002.25 ETB/ha was obtained from Debark-1 at the 12 days HA and the lowest 941,201.13 ETB/ha was recorded at Tila variety on the 6 days HA. Except for LSR and DM, all morphological and chemical compositions have a positive correlation. From the study, it can be concluded that based on FFBY, DMY, CP, and economic benefits Debark-1 was the recommended variety on the 12 days HA, followed by HB-1307, Local, and Tila investigated in the current study.