Production of tomato in Ethiopia as well as in Amhara Region is low; this could be due to lack of high yielding and high quality genotypes. Therefore, the aim of this study was to evaluate variability and performance of 17 tomato genotypes for growth, yield and quality at SARC Kobo sub center during the 2021 irrigation season. A field experiment was laid out in Randomized Complete Block Design (RCBD) with three replications. Analysis of variance for 21 quantitative traits revealed that there was a highly significant difference (P<0.01) among the seventeen genotypes for all the characters studied except unmarketable yield. This indicates the presence of considerable genetic variability for further improvement of tomato for yield and quality aspects. The difference between PCV and GCV was relatively small for most of the traits, implying that genotype contributed more than environment in the expression of the characters. High GCV and PCV was observed for titratable acidity, average single fruit weight, physiological weight loss, plant height and number of cluster per plant. High heritability estimates coupled with high values of genetic advance as percent of mean observed among some traits; indicates that they were governed by additive gene action and therefore provides the most effective condition for selection. Metadel, Woyno, Fetan, and Miya tomato genotypes were matured earlier than the rest of the tested genotypes. Chali (59.62 t/ha), Eshet (51.95 t/ha), Metadel (51.12 t/ha) and Melka salsa (47.43 t/ha) and tomato genotypes gave better marketable fruit yield while Chali, Eshet, Gelila, Metadel, Bishola, and ARP D2 Tomato genotypes had the highest juice content. From the participatory evaluation, Metadel, Chali, Eshet and Cochiro were selected by the farmers. In terms of marketable yield; Chali, Eshet, Metadel, and Melka Salsa genotypes can be recommended for the study area. In terms of fruit quality parameters, the genotypes Chali, Eshet, Gelilal, and Metadel can be suggested for commercial production and processing industry. However, it is advisable to repeat the experiment on different agro-ecologies and years with more number of materials.