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Microbial Load and Antibiogram of Escherichia Coli in Raw Milk from Dairy Cows In North Mecha District, Northwest Ethiopia.

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dc.contributor.author Yeshi Telay
dc.date.accessioned 2023-02-23T09:15:22Z
dc.date.available 2023-02-23T09:15:22Z
dc.date.issued 2022-07
dc.identifier.uri http://ir.bdu.edu.et/handle/123456789/15098
dc.description.abstract Milk and milk products have important role in feeding the rural and urban population owing to its high nutritional value. However, it is subjected to contamination whenever there are poor hygienic practices. A cross sectional study was conducted to determine the microbial load of E.coli and its antimicrobial susceptibility pattern from raw cow‟s milk in North Mecha district from December 2021 to July 2022. One stage cluster sampling method was used to collect pooled raw milk samples from selected households. A total of 180; 60 milk, 60 hand and 60 equipment swab samples were collected hygienically and processed to investigate E.coli by using conventional culture method. Escherichia coli were isolated from 41.7, 13.3 and 11.7% of milk, milking equipment and hand swab samples respectively. The overall prevalence of E.coli was 22.2%. The occurrence of E.coli was significantly associated with farm hygiene and smoking of milking equipment‟s. The mean count of E.coli from raw milk was 3.76±1.16 log 10 CFU/mL,with minimum and maximum value of 1.48 and 5.69log CFU/mL respecti vely. All isolates (40) of E.coli were resistance to ampicillin and erythromycin. Whereas, 95, 92.5, 65 and 55.5% of E.coli isolates were susceptible to gentamicin, chloramphenico l, nalidicic acid and co-trimazole respectively. Based on this finding, only gentamicin and chloramphenicol were the most effective treatments for E.coli, while nalidicic acid and co-trimazole were not working at normal dose. This finding concludes that there was a gap on hygienic practices and E.coli count exceeded the limits of food safety standards. Therefore, every personnel engaged in all dairy activities need to be gain training about the whole sanitation and hygienic practices. 10 en_US
dc.language.iso en en_US
dc.subject Veterinary Public Health en_US
dc.title Microbial Load and Antibiogram of Escherichia Coli in Raw Milk from Dairy Cows In North Mecha District, Northwest Ethiopia. en_US
dc.type Thesis en_US


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