Biomedical waste consists of toxic or hazardous substances and it is the second most hazardous waste in the world, just after radiation waste; so, it should be managed safely and appropriately. Generation and disposal of biomedical wastes have become an emerging problem worldwide. It is regarded as one of the most significant urban decision-making issues and the foremost problem in healthcare institutions. Accordingly, the study aimed to assess biomedical solid waste management practices and the variables influencing them in public and private healthcare facilities of Bahir Dar City, Ethiopia. A cross-sectional institutional analysis was performed in 14 public and private HCFs, selected through stratified followed by purposive sampling; and 85 key informants were selected using convenience sampling in each healthcare facility independently. Data were collected using questionnaires, interviews, and observational checklists. The data were managed and analyzed by STATA version 17. Multivariable binary logistic regression analysis was used to identify factors associated with BMWM practices based on the adjusted odds ratio (AOR) with a 95% confidence interval (CI) and p-values<.05. The results showed that the surveyed HCFs had 55.3% good BMWM practices; out of which, 64.4%, 60.0%, and 36.4% were hospitals, health centers, and specialty centers respectively. As well as public and private HCFs were counted as, 52.9% and 58.8% of good practice respectively. BMWM practice in the surveyed overall HCFs was significantly associated with the segregation of waste by health professionals according to guidelines (AOR= 6.82, 95% CI: 1.85, 25.08), availability of treatment equipment (AOR= 4.80, 95% CI: 1.34, 17.15), and presence SOPs (AOR= 0.24, 95% CI: 0.06, 0.10). The annual estimated waste generation rate of the surveyed overall HCFs was 789.14 tones/year; out of which, hospitals, health centers, and specialty centers scored (632.91, 124.47 & 31.76) tons/year respectively. Similarly, public and private HCFs were recorded (862.87 & 140.79) tons/year respectively. Except for specialty centers, the HCWM of all the surveyed HCFs had more than half good practices. This study demonstrated that BMWM in healthcare facilities is still challenging. Medical facilities, furthermore specialty centers should enhance their HCWMPs by encouraging procedures that guarantee appropriate waste management methods under guidelines from segregation to disposal.