dc.description.abstract |
Mastitis is a multifactorial disease of mammals that has substantial implications on
production, welfare, and public health, but it is far less studied in goats than in cows. A
cross-sectional study with simple random sampling at the lowest unit was conducted in
selected districts of Northeastern Amhara, Ethiopia, from September 2024 to May 2025.
The objectives of the study were to assess knowledge, attitudes, and practices (KAP) levels
of herders on mastitis in goats, estimate its prevalence, assess associated risk factors,
identify bacteria, and evaluate their antimicrobial susceptibility. A semi-structured
questionnaire survey was utilized to evaluate KAP levels, while a physical examination and
the California mastitis test were employed to examine mastitis in goats. Laboratory tests for
bacterial identification and antimicrobial resistance were conducted following standard
protocols. Stata17 and WHONET software were used for statistical analyses. Out of the 275
survey participants, 30.68% reported mastitis incidents in goats during the study period. The
average scores for knowledge, attitude, and practice related to mastitis were 47.78%,
69.39%, and 46.18%, respectively. The majority of respondents did not attend school, and
their knowledge of causes of goat‟s mastitis, zoonotic diseases, and subclinical mastitis was
significantly associated with district and age demographics. Out of the 397 lactating goats
examined, mastitis prevalence was 24.74% at goat level (CM: 2.52%, SCM: 22.22%) and
16.60% in udder halves (CM: 1.39%, SCM: 15.21%). Multivariable logistic regression
analysis revealed that lactation stage (OR=1.92; 95% CI: 1.89-6.30), districts (OR=3.47;
95% CI: 1.01-3.65), parity (OR=2.73; 95% CI: 1.11-6.67), and mastitis history (OR = 13.6;
95% CI: 4.64-40.15) (p<0.05) were statistically associated with the occurrence of goat
mastitis. Upon bacterial isolation and identification, the most common bacterial isolate was
Staphylococcus (39.83%), followed by E. coli (10.17%), Enterococcus, Micrococcus, and
Klebsiella (all < 5%). Staphylococcus was resistant to ampicillin and tetracycline, with a
multidrug-resistant (MDR) prevalence of 21.16%, whereas E. coli was resistant to
ampicillin, with an MDR prevalence of 8.3%. The overall MDR prevalence was found to be
17.31%. In conclusion, herders lacked good knowledge and practices about mastitis and
zoonotic risks, which most likely contributed to the high prevalence of mastitis and antimicrobials -resistant bacteria. As a result, tailored training on the causes of mastitis and
zoonotic risks, control and prevention for goat farmers, and further research on bacterial
species and resistance factor identification are recommended. Furthermore, kanamycin and
gentamycin were found to be effective against staphylococci and thus recommended for use,
while tetracycline and streptomycin were found to be effective against E. coli and thus
recommended. |
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