On Mount Guna higher land degradation particularly soil erosion problems has a deleterious impact on soil fertility and depletion of soil organic carbon stock due to land use types and slope gradient varation. Land use type, slope gradient and soil depth have different impacts in soil properties and soil organic carbon stock variation either positively or negatively. Therefore, the study was conducted to evaluate the effects of land use and slope on soil organic carbon stock and soil properties of Mount Guna, northwestern highland of Ethiopia. LULC map was done from 30 m x 30 m resolution satellite imagery of 2021 which is freely downloaded from USGS Earth Explorer and the classification process were done using Arc GIS 10.8 version. Stratified sampling technique was employed. A total of 144 composite soil samples were collected from 0-15 cm and 15-30 cm soil depth under the major land use types across three slope gradients with three replication by auger for selected soil physicochemical analaysis, of which 54 soil samples from developmental, 54 from buffer and the rest 36 soil sample was from core zone. Correspondingly, 144 soil samples were collected for soil BD and soil moisture content determination by core sampling method. For data analysis, the general linear model procedure of SAS version 9.4 was used. The result showed that, land use types, slope gradients, soil depth and land use type interact with slope classes had very highly and highly significant difference on selected soil parameter and soil SOC stock within developmental, buffer and core zones. The highest sand fraction, percentage of silt, BD and Av. P were recorded under cropland within developmental and buffer zones. Similarly, within the core zone, except Av.P which was higher under natural forest land, all other listed parameters were higher on Guassa grass land, whereas the highest clay percentage, SMC, pH, TN, CEC, OC and SOCS were recorded under the natural forest land within each zone. In terms of slope classes, except sand and BD all other parameters were increased with decreasing slope gradient within each zones. With regarding to soil depth the sand fraction, Av. P, TN, CEC, OC and SOCS decreased with increasing in soil depth, while the clay content, BD, SMC and pH were increased with increasing soil depth within developmental, buffer and core zones. Regarding with interaction effects, the highest clay, pH, TN, CEC, OC and SOCS were observed on the lower slope under natural forest land, while the lowest was recorded on the upper slope classes of crop land within developmental and buffer zone and under Guassa grass land within the core zone. SOCS was decreased with increasing slope, meaning SOCS had a negatively relationship with slope gradients. Generally land use type, slope gradient, soil depth and in some extents by interaction cause variations in physicochemical properties of the soil and SOCS storage of the study area.