Sesame, often called the "Queen of Oilseeds," is highly resistant to oxidation and rancidity. It is Ethiopia's second-largest export after coffee. However, oilseed crops can be substrates for molds that produce harmful substances, such as aflatoxins and ochratoxins. Limited information exists on mycotoxin occurrence in sesame. This study aimed to evaluate the total aflatoxins (AFT) and ochratoxin A (OTA) content in sesame seeds stored by farmers in Northwest Ethiopia. Sesame samples were collected from farmers' households in Northwestern Ethiopia during two time intervals, totaling 100 sesame seed samples. The ELISA test kit was used to analyze AFT and OTA levels in the samples. Standardized questionnaires assessed farmers' knowledge, attitudes, and practices about mycotoxin contamination and the associated health risks. The physical quality parameters of sesame seeds were determined by laboratory analysis. Data were analyzed using descriptive statistics, ANOVA, T-test, and correlation analysis using Pearson’s correlation. Moisture content ranged between 2.81±0.32 and 4.10±0.36, water activity between 0.38±0.01 and 0.45±0.03, thousand kernel weight between 2.66±0.16 and 3.08±0.33, bulk density between 0.627 ± 0.01 and 0.663±0.01, mold infection between 5.65±3.18 and 16.50±5.60%, and grain impurity between 1.10±0.46 and 2.06±1.46. The L*, a*, b* color values ranged from 62.23±2.50 to 65.40±1.88, 4.03±0.73 to 4.47±0.72, and 21.66±1.63 to 23.15±0.09, respectively. Total Aflatoxins (AFT) and OTA levels in positive samples ranged from 2μg/k to 64 μg/kg, and 2μg/kg to 5.9μg/kg, respectively. There was a co-occurrence of AFT and OTA in 20% of sesame samples. Key Words: Total Aflatoxin, Ochratoxin A, KAP, ELISA, sesame seed,storage practic