Bioethanol is an interesting biofuel made of renewable biomass alleviating energy crisis and environmental pollution that arise from fossil fuels. Fruit wastes are renewable biomass that are rich in sugars which can be used for bioethanol production. The goal of this study was focused on production and characterization of bioethanol from mixed mango and pineapple peels. Bioethanol production involves several steps such as pretreatment process, hydrolysis process, fermentation, and distillation steps. The proximate and chemical composition of both raw and pretreated mango and pineapple peels were performed with ASTM protocols. Pretreatment process was applied to evaluate the effect of microwave power (100, 300 and 500 W) and irradiation time (2, 6 and 10 min) using CCD. The results showed the optimum conditions at 300 W and 6 min yielding 82.35% and 80.05% lignin removal from mango and pineapple peels, respectively. The FTIR analysis was conducted to confirm the chemical changes of mango and pineapple peels before and after treatment. After pretreatment, the acid hydrolysis process was applied to optimize the effect of H2SO 4 concentration (0.5, 1.75 and 3%), hydrolysis time (30, 60 and 90 min), and biomass mixing ratio (25%, 50% and 75%). The results showed the best condition at 1.75% H 2SO 4 , 50:50% mixing ratio and 60 min hydrolysis time obtaining 51.7 g/L reducing sugar yield, which was measured by DNS assay. Fermentation process was done for 48, 72 and 96 hr . The maximum ethanol yield of 42.22% (v/v) was achieved at 72 hr. FTIR peaks associated with O-H and C-H stretching and vibrations confirmed the presence of ethanol in the product. This study’s result confirmed that mixing mango and pineapple peels boosts bioethanol yield than using single peels. Finding from this research work portray that mango and pineapple peel can be utilized as the potential substrate for bioethanol production. Keywords: Bioethanol; Characterization; Hydrolysis; Mango peel; Optimization; Pineapple peel; Pretreatment