Bovine herpesvirus-1 (BoHV-1) causes infectious bovine rhinotracheitis (IBR), and infectious pustular vulvovaginitis (PV) in cows and infectious balanoposthitis (IBP) in bulls. Bovine herpesvirus-1is highly contagious cattle disease, which causes significant reproductive losses in the dairy industry worldwide. A cross-sectional study was conducted using a multistage stratified sampling method between April 2022 to December 2022 to estimate the seroprevalence of BoHV-1 in dairy cattle and its associated risk factors in Bahir Dar city and Bahir Dar Zuria district, northwest Ethiopia. A total of 384 serum samples from 121 herds of cattle aged above 6 months reared in rural, peri-urban, and urban dairy production systems were collected. Competitive ELISA assay was performed to detect antibodies directed against the gB glycoprotein of BoHV-1. Besides, a semi-structured questionnaire was administered to dairy cattle producers to obtain data on household demography, dairy cattle production system, animal level and herd level management practices. The present study revealed that the overall animal level and herd level seroprevalences of BoHV-1 were 63.54% (95% CI: 58.8–68.2) and 81.82 % (95% CI: 74.8–88.7) respectively and varied between production systems. The overall animal level prevalence of BoHV-1 was significantly higher in rural dairy production systems (AOR=4.2, 95% CI: 1.78–9.85; p<0.001) than in peri-urban (AOR=2.2, 95% CI: 1.13-4.42; p=0.019) and urban dairy production systems. Age was found one of the significant risk factors affecting the prevalence of BoHV-1 infection (P<0.001). Animals aged above five years (>5 years) were 3.2 times (AOR=3.2, 95%CI: 1.76–5.86; P<0.001) more likely to be affected by BoHV-1 compared to cattle below two years (< 2 years). Regarding the origin, Purchased cattle were more likely at risk of acquiring BoHV-1 (AOR=2.9, 95% CI: 1.57–5.52; p=0.001) as compared to homebred cattle. The prevalence of BoHV-1 was also significantly influenced by parity (OR=2.9; p=0.020) in which multiparous cow were more affected by BoHV-1 compared to primiparous cows. Furthermore, a significantly higher prevalence of BoHV-1infection was recorded in cows with a history of reproductive disorders. Likewise, the prevalence of BoHV-1infection was significantly higher (OR=4.1, 95% CI: 2.17–7.7; p<0.001) in animals with a history of respiratory disorders when compared to animal with no history of respiratory disorder. In conclusion, this study revealed that the prevalence of bovine herpes virus-1 (BoHV-1) was found to be higher in the present study area. Besides, a multitude of host and management risk factors affecting the prevalence of BoHV-1 infection were investigated. Such higher prevalence could significantly affect the productivity and overall production of dairy herds in the study areas. This necessitates evidence based integrated BoHV-1 control and prevention strategy in the study area.