A field experiment was conducted on the Vertisols of Debre Zeit Agricultural Research Center (DZARC) and Chefe Donsa experimental sites, central highlands of Ethiopia to evaluate the effects of nutrient omission on yield and yield components of durum wheat. The experiment was laid out in a randomized complete block design with ten treatments and three replications. The treatments were Control, Recommended NP, PKSZnB (-N), NKSZnB (-P), NPSZnB (-K), NPKZnB (-S), recommended NP + S2, NPKSZn (-B), NPKSB (-Zn) and NPKSZnB. The soil analysis results before treatment applications showed neutral pH, clay texture, low in total N, B, OC, BD and high in available P, and Zn, exchangeable K, and CEC and medium in available S for both sites. Yield and yield attributing characteristics of durum wheat such as plant height, spike length, tiller numbers, and grain, straw and biomass yields, and agronomic efficiency, partial factor productivity and uptake of nitrogen and phosphorus were significantly influenced by the nutrient omission treatments, especially minus N. As compared to the treatment receiving all six nutrients, the omission of N, B, and the existing rec. NP reduced the grain yield by 57, 19, and 18%, respectively at the DZARC and at Chefe Donsa the omission of N, Zn, and the existing rec.NP reduced the grain yield by 68, 21, and, 20%, respectively. From the study a large reduction in the grain yield was observed in the treatments which had no N nutrient indicating that nitrogen is the most yield limiting nutrient for durum wheat production in the study areas. Based on the obtained result , the Ada'a and Gimbichu districts are both prospective locations for the cultivation of durum wheat and the deficiency of macro- and micronutrients had an impact on yield reduction. Since this is one year of data, further research is required on farmers’ fields.