Soil organic carbon (SOC) and total nitrogen (TN) stocks are essential for climate change mitigation and agricultural production. The study was conducted in 2022 to evaluate SOC and TN stocks variation under different land-use types, slope gradients, and soil depths in Aregedif micro-watershed of the western Gojjam zone, northwestern Ethiopia. For this study, stratified random sampling with a 10 m by 10 m size of 9 plot in each land-use was marked to collect soil samples in an "X" design. As a result, 81 composite and core sampled soils from three land-use types, three slope gradients, and three subsequent soil depths were collected in three replications. The slope gradients and land-use types were identified with a DEM of 30 m x 30 m resolution and a satellite Sentinel 2 of 10 m x 10 m resolution, using ERDAS imagine 2015 and the map was developed using Arc GIS 10.7. The data was analysis using Microsoft Office Excel 2007 and Statistical Package for Social Sciences (SPSS) software version 23. After comparing the means a multivariate ANOVA was conducted for significant differences. Moreover, a mean separation was made using one way ANOVA LSD test. The result confirmed that SOC and TN stocks were significantly (p<0.05) influenced by land-use types, slope gradients, and soil depths. The highest mean value of SOC and TN stocks was observed on grassland (25.11±2.08 t/ha, 1.98±0.17 t/ha), flat to gentle slopes (22.27±1.55 t/ha, 1.96±0.14 t/ha) and 0–10 cm soil depths (24.56±1.99 t/ha, 2.28±0.14 t/ha) while the lowest value of SOC and TN stocks was observed on cultivated land (14.16±1.68 t/ha, 1.44±0.14 t/ha), steep slopes (14.35±1.63 t/ha, 1.28±0.13 t/ha) and soil depths of 20 –30 cm (13.58±1.30 t/ha, 1.10±0.11 t/ha) respectively. This could be due to the frequent addition of OM in grassland and low-input agricultural systems in cultivated land and the erosion movement of C and N containing OM in flat to gentle slope, and the removal of organic materials from steep slope by runoff and erosion. furthermore, the top layer accumulates more OM than the lower one. There was a strong positive linear relationship between SOC and TN stocks (R 2 =64.0). Based on the confirmed result, different land-use types, slope gradients and soil depths have different potentials to change SOC and TN stocks either positively or negatively. Therefore, integrated land management practices are the most effective way to increase SOC and TN stocks.