The study was conducted in Lasta Lalibela district North Wollo zone, Amhara region with the objectives, to identify the major honeybee flora and their phenology through beekeepers interview, field observation and honey pollen analysis. To do this, two representative agroecologies, mid land (two Kebeles) and low land (three kebeles) were selected using stratified random sampling procedures. A total of 172 Beekeepers were randomly selected and interviewed using a structured questioner to collect relevant information. In addition 45 quadrates were established in different land use system: protected area (20), farm land (15) and back yard (10) by means of transect sampling method to collect vegetation data including bee flora species diversity and abundance. Moreover, 20 honey samples each weighing a minimum of 500 gm was collected from beekeepers during major honey flow or harvested seasons (October to November). According to the survey results the majorities 92.4% (159) of the respondents were males and 7.6 % (13) were females. During the interview the beekeepers were asked to identify the major and minor honey bee floras and flowering period in different agro-ecological zones. This study shows a total of 49 commonly grown bee flora species belonging to 25 families were identified. Accordingly, the major bee flora species in the study area includes Biden spp, Becium grandiflorium, Euclea schimperi, Hypoestes triflora, Cordia africana, Trifolium Spp, Acacia spp, Eucalptus spp and Unidentified species. Furthermore the present finding showed that 55.8%, 34.2%, 29.8%, 18.8% and 5.3% of the existed bee floras belonging to herbs, shrubs, trees, crop and grass, respectively. A total of 11 pollen and nectar source flowering plant species belonging to 6 families were identified from honey pollen analysis. The pollen analysis from honey samples revealed that, 89.7%, 88% and 69.18% of the pollen in honey found to be Trifolium semipilosum, Bidens spp and Echinops spp, respectively. The present study shows pests and predators are the major constraints of beekeeping practice followed by, drought and unwise pesticide application. Therefore to solve this problem, provision of dearth period supplementation and growing multipurpose and drought resistant bee forage plants shall be practiced. Further study is recommended on honeybee colony carrying capacity of the district.