Background: Anemia is a public health problem affects low, middle and high income countries has adverse effects on health consequences and impacts on social and economic development. Globally, 38.2% of pregnant women were anemic, of this South- East Asia, Eastern Mediterranean and African regions accounts 38.9% to 48.7%. In developing nations almost two-third of pregnant women are affected by anemia and in Ethiopia 29.1% of pregnant women were anemic However, literatures are limited, therefore, this study aimed to investigate the prevalence and associated factors of anemia among pregnant women attending antenatal care Unit in Buldiglu District, northwest Ethiopia. Objective: To determine the prevalence and associated factors of anemia among pregnant women attending antenatal care in Buldiglu District, benishangul Gumuz western Ethiopia 2020. Method: Institutional based cross–sectional study was conducted to assess the prevalence and associate factors of anemia in pregnant women attending antenatal care in Buldiglu district from 1st march to 4th may 2020. The study participants were recruited by using systematic random sampling method. The data were collected using structured interview based questionnaire and a finger prick blood sample to determine hemoglobin level. Result: The overall prevalence of anemia among pregnant was 24.5%; Of this 22% and 78% had moderate and mild anemia respectively; But there is no sever anemic pregnant. pregnant women who had well-nourished (MUAC≥21cm). were less likely to develop anemia during pregnancy by 80% [AOR= 0.201 (95% CI 0.065, 0.622)] as compared to with under nourished (MUAC≤21cm). Conclusion: The overall prevalence of anemia among pregnant women attending antenatal care in the study area was 24.5% which are found to be a moderate public health problem. Nutritional status were significantly associated with anemia in pregnant women. Recommendation: further large scale longitudinal studies should be done in respect to the importance of regular visit to maternal care centers and health education promotion programs regarding the cause and prevention of anemia among pregnant women by assessing micronutrients and other causal related factors for anemia.