This study was conducted in Tehulederie district, Eastern part of Amhara National Reginal State to characterize the beekeeping system, analyze the potentials and constraints for beekeeping and suggest possible solutions for existing problems, identify major honeybee flora and their flowering periods, and evaluate the major honey quality in different agroecology of the study area. Data were collected from 120 beekeepers having bee colonies and living in three different agro-ecologies. The study had two parts: part one was data collection among beekeepers with a semi-structured questioner by single- visit-multiplesubject formal survey method. All the collected data was analyzed by using SPSS v-20 Duncan's and one-way ANOVA method. From the total 120 sample beekeepers 92.5 % of them were male headed households, 95.8 % of them are married, mean age of the respondents was 48. 97±11. 03 years and they owned 105, 17 and 57 traditional, transitional and frame hived colonies respectively. The study result indicates that based on their level of technological advancement, three distinct types of beekeeping practices were used by the sample beekeepers in the area. These are traditional (local) hive based, transitional (Ethioribrab top bar) hive based and moveable frame (box) hive based beekeeping practices. Most of the beekeepers in the study area kept both traditional, transitional and frame beehives at their eave of the house, only 10.8% feed their colony at dearth, 95.5% of them increases colonies by catching the swarm colonies. The mean honey yield of traditional, transitional and framed type hives was 5.64, 12.7 and 16.9 kilogram per year, respectively and all of the respondents sell the unstrained honey directly to local market. Drought or rain dependent of the agriculture (76.7%), increased cost of production (75%), pests and predators (47.5%), application of pesticides and herbicides (43.3%) and lack of bee forages 32.5% are the major constraints to tackle the development of beekeeping in Tehulederie district. The second part of the study was the determination of honey quality produced in the study district. Twenty four honey samples were collected from crushed comb (traditional and transitional hive) honey and framed hive honey as two distinct groupsfrom the represented 3 VIII different agro-ecologies of the district directly from the apiary farm gates with tightly closed half a kilogram of plastic containers analyzed for eight honey quality parameters (moisture content, total reducing sugar, pH, acidity, diastase activity, sucrose content, HMF and mineral content) in the Food Chemistry and Analysis laboratory of School of Chemical and Food Engineering, Bahir Dar University, Ethiopia. The mean moisture content, mineral content, acidity, pH, HMF, diastase activity, sucrose content, and total reducing sugar, are 16.7%, 0.07%, 22.3 meq. acid/kg, 3. 85±0.46, 37.7 mg/kg, 14.4 Goth scale, 4.04 % and 64.3meq/kg respectively. All the eight determined parameters showed that 100 % of the sample means were situated in the acceptable range of the world honey quality standard set by Codex Almentarious, 2001. Package designing for implementation of improved practices, gaining of efficient seasonal trainings, plantation of drought tolerant bee forages, establishment of diversified beekeeping products collection and processing centers, integrating the responsible crop scientists, animal science experts and other administration organizations for efficient utilization of agrochemicals and farther study are recommended to enhance the sector.