This study aims to identify organizational culture that is important for the transfer of knowledge in six selected regional public organizations of Amhara Region. The study deploys explanatory and cross-sectional design and adopts a quantitative approach. After a literature review, five key organizational cultures (collaboration, learning & development, management support, trust and shared vision) were identified as predictors of knowledge transfer. From a total of 1,106 employees in selected six public organizations, 294 were randomly selected and participated in the study. Primary data was collected via a closed-ended questionnaire. Descriptive, correlation and multiple regression statistical tools were used to analyze the data and draw conclusions. The result of this study reveals that knowledge transfer is significantly influenced by collaboration, learning & development, management support, trust, and shared vision. Management support, however, generates the highest effect on knowledge transfer performance. Thus, this research recommended public organizations to give more attention to improve management support practices. In addition, since organizational elements are complementary and people are the most important component, public organizations need an integrated and people-centered knowledge management strategy.