Striga is major biotic constraint and a serious threat to sorghum production in areas of semi- arid tropics. The objectives of this study were to evaluate the performance of 49 sorghum genotypes including resistance and susceptible checks to Striga hermonthica and to investigate the relationship among yield and Striga related traits. The experiment was conducted at Kobo research sub-center, North Eastern Ethiopia in 2018 main cropping season using simple lattice design. The analysis of variance revealed significance difference among the genotypes for all traits including grain yield, days to maturity, plant height and striga count ranged from 1462- 7972 kgha-1, 112-130days, 120-285cm and 3.35-34.25striga counts, respectively. Genotypes ETSC-14118-2-1, ETSC-14019-9-1, ETSC-14184-8-3, ETSC-14019-14-2, ETSC-14127-1-3, ETSC-14018-1-3, ETSC-14217-10-1, host low number of Striga count whereas, ETSC-14220-1-3, ETSC-14124-6-1, ETSC-14124-8-3, Susceptible check, ETSC-14196-1-3 and ETSC-14126-5-2 comparatively showed high Striga severity. The genotypic coefficient of variation (GCV) ranged from 2.97% for days to maturity (DM) to 24.94% for grain yield, while phenotypic coefficient of variation (PCV) ranged from 2.26% for DM to 33.34% for biomass (BM). GY recorded high GCV, medium for plant height and thousand kernel weight whereas while GY, HW and BM recorded high PCV. Heritability values were ranged from 12.4% to 99.8%. Phenotypically positively significant traits were head weight(0.79*),head number(0.45*),panicle width(0.29*),and panicle length(0.26)Whereas, phenotypically and genotypically negative significant (P ≤ 0.05) for DF (rp = -0.28, rg = -0.32), DM (rp = -0.27, rg= -0.28) and Stm-2( rp = -0.36, rg = -0.61),SV(rp = -0.48, rg = -0.73) and SSV (rp = -0.37, rg =-0.69 ), respectively. The first seven PCA explained 89.5% of the total variation and the traits plant height (0.83), Striga severity (0.71) days to maturity (0.59), panicle length (0.56) and days to flowering (0.54) accounted for most of the variability. Six clusters were found and significant distances were observed among cluster IV and V, and III & IV. Accordingly, resistance, tolerance and susceptible genotypes were identified. However, further research is needed to test these genotypes to prove the current results. Keywords: Cluster analysis, Correlation, Grain yield, Principal component, Striga hermonithica