ABSTRACT The main objective of this study was to assess the effects of human disturbance on the abundances, and distribution of some selected species of large mammals in Awash National Park. The study was conducted from October 2017 to February 2018. The study site was categorized into low disturbance, intermediate disturbance, and high disturbance level based on the status of ground covers, number of cattle, sheep, and goat encountered. Twenty transect lines were assigned for each level of disturbance. The length of transect line was 500m while the distance between subsequent transect lines were assigned based on its visibility in different habitat types. During walking along the transect line, the selected large mammals species, number of individual, habitat type, type of human activities encountered were recorded. There was statistically significant mean difference in the encounter rates of livestock grazing (p=0.019, F (2, 57) = 4.233) and in tree cutting (P=0.040, F (2, 57) = 3.416) across the three level of human disturbance. There was statistically highly significant variation in the proportion of each ground cover (χ2=85.14, p=0.001, df = 12) across the three level of human disturbance. There was statistically significant mean difference in the encounter rates of Anubis baboon (Papio anubis) (p=0.032, F (2, 36) = 3.796). However, there were statistically insignificant mean difference in the encounter rates of Beisa oryx (Oryx beisa beisa) (p=0.171, F (2, 57) =1.820), Soemmering’s gazelle (Gazella soemmeringi) (p=0.952, F (2, 57) =0.049), and Hamadryas baboon (Papio hamadryas) (p=0.974, F (2, 56) =0.026). Cattle grazing had significant effects (p=0 .019, df=1) on the abundance of Beisa oryx and highly significant effects (p<0 .001, df=1) on the abundance of Anubis baboon. But, none of human activities encountered affected the abundances of Soemmering’s gazelle and Hamadryas baboon significantly (p>0.05, df=1).There were statistically insignificant variation in mammalian distribution across the three disturbance level (χ2 = 1.21, p = 0.97 df = 6) and four habitat type (χ2=5.093, p=0.973, df=12). In conclusion, human activities such as livestock grazing and trees cutting were the major factor which affects the ground covers, mammalian abundance, and distribution in the park. Therefore, actions that limit these human activities in and around the park are recommended to ensure a more sustainable future for the large mammals and their in ANP. Keywords/Phrases: Anubis baboon, Awash National Park, Beisa oryx, Hamadryas baboon, human disturbance, Soemmering’s gazelle