This study examined the effect of humanitarian logistics practices on the organizational performance of Amhara National Regional State (ANRS) Disaster Prevention and Food Security Program Coordination Office in Ethiopia. The research was spurred by the growing complexity of humanitarian crises and the vital role of logistics in delivering fast and effective disaster relief. The study specifically examined the effect of four core logistics practices (procurement, transportation, inventory management and information communication technology) on the organizational performance in terms of responsiveness, lead time, reliability and service quality. The study adopted a quantitative research approach with descriptive and explanatory designs. Primary data was collected from employees of the organization using structured self administered questionnaires. Descriptive statistics, correlation and multiple regression analysis were used to analyze the data. The results show that all four humanitarian logistics practices have a positive and statistically significant effect on organizational performance. ICT and procurement practices were found to have the highest influence among these, followed by transportation and inventory practices. The study also identified operational challenges like delays in procurement processes, poor transportation infrastructure, weak inventory control systems and limited use of ICT, which had a negative impact on efficiency and effectiveness. The findings underscore the need to integrate logistics functions and build institutional capacity to enhance performance. The results indicate that better humanitarian logistics practices can enhance organizational performance in disaster response operations. It recommends that the organization should invest in up-to-date ICT systems, strengthen transport networks, improve procurement processes and adopt sophisticated inventory management techniques for timely and effective service delivery. This study contributes to addressing the empirical gap in humanitarian logistics practices and organizational performance within the Ethiopian public sector context, particularly in the ANRS Disaster Prevention and Food Security Program Coordination Office. The findings also provide practical insights for policymakers, practitioners, and future researchers to improve humanitarian logistics operations and organizational performance.