The present study was designed to compare the effects of aerobic and anaerobic training on physiological parameters such as (vo2max, SBP, DBP, RHR), morphological variables such as (BMI, %BF, WHR), hematological indexes such as (RBC, WBC, HCT, HGB, PLT) and biochemical markers such as (LDL, TC, TG, BG) of sedentary adult office workers. This study was performed as a true experimental study (randomized control two-group design), in which subjects were voluntarily selected. There were 32 participants, aged between 20-39 years. Subjects were grouped into aerobic training (n=16) and anaerobic training (n=16) randomly. Aerobic training was given for three days a week at 60-75% of maximum HR for 8 weeks. Anaerobic training was given for three days a week at 75% of 1 RM and 80-90% of MHR for 8 weeks. Data were presented as mean ± standard deviation or mean change with 95% confidence intervals. The subject’s physiological, morphological, hematological, and biochemical markers were measured before and after exercise. The two-tailed paired sample t-test was carried out to calculate the mean differences between pre-and post-phase variables within the same group. An Independent sample t-test was used to compare the effects of the two training groups on the dependent variables. A partial eta-squared (ηp 2) was used as a measure of effect size, how much is the difference between aerobic and anaerobic training on dependent variables? After the exercise program, there were meaningful decreases in physiological variables in both intervention groups except vo2max. In the case of vo2max, there was a meaningful increase in both intervention groups. The morphological variable results there was a meaningful decrease in both intervention groups. In addition in the hematological indexes results of the anaerobic exercise group, some meaningful increases were determined in the value of PLT and HGB. However a meaningful decrease in WBC and a slight decrease in RBC and HCT value. Whereas in the aerobic exercise group, a meaningful increase was determined in the value of PLT, HGB, RBC, and HCT, however, there were meaningful decreases in WBC. In The biochemical marker results, there was a meaningful decrease in both intervention groups. There is no significant difference between aerobic and anaerobic training on the change in physiological, morphological, hematological, and biochemical markers of sedentary adult office workers. Whereas, aerobic training is more beneficial than anaerobic training in improving physiological (SBP, DBP, and RHR), morphological (BMI, WHR, and %BF), hematological (RBC, PLT, HCT, and HGB), and biochemical (LDL and BG) indicators. Whereas anaerobic exercise has been the most effective type of exercise by showing a significant lowering effect on TC and TG and hematological markers on (WBC). And also shows a significant increment effect on vo2max of sedentary adult office workers.