This work presents Synthesis, characterization and biological investigation of new mixed complexes of zinc (II) metal containing resorcinolate ligands. The synthesized complex was characterized by different analytical and spectroscopic techniques such as UV-visible spectroscopy, Fourier transform infrared spectrometry (FTIR), chloride determination, Thermo gravimetric (TG) analysis, melting point determination, pH, solubility tests and molar conductivity. From ligand and metal estimation data the stoichiometry of the complexes was found to be 4:1 (ligand: metal) having the general formula Na 2 [Zn(HR) ] with a tetrahedral geometry. Based on the analytical and the complex was evaluated for its biological activity on two Gram-positive bacteria (Staphylococcus aureus and Staphylococcus epidermidis ) and two Gram-negative bacteria (Escherichia coli and Klebsiella pneumoniae). It was found biologically active on the Gram-positive bacteria but showed no activity against the Gram negative ones. This complex showed better activity compared to the ligand but lower than the salt and the reference. Therefore, this complex could be used as an antibacterial agent against the tested Gram-positive bacteria after cytotoxicity investigations. 4