Water hyacinth (Eichornia crassipes) is a hazardous and fast-growing invasive aquatic species for which international concern has been risen due to its environmental influence. Textile industries are released large amount of toxic and hazardous pollutant of dyecontaining wastewater that highly degrade the environment. The aim of this study was removal of Azo-dye from textile wastewater by graphene composite bio-char synthesized from water hyacinth stem. Temperature (350, 450, and 550℃), and time (30, 40, and 60 min) were considered as a factor for the synthesis of graphene-composite biochar using pyrolysis process and oxidation reaction. Temperature (25, 30, and 35 ℃ ), pH (4, 7, and 10), and flow rate (3, 4.5, and 6 ml/min) were also considered as factors to analyze the adsorption efficiency of graphene composite biochar for azo-dyes removal from textile industries effluents using fixed bed column. The characterization of graphene-composite biochar was performed using FTIR, SEM, XRD, and BET surface area. In this study, the maximum adsorption efficiency of graphene-composite biochar (99.91%) was found at pH-10, 25℃, and 3ml/min whereas the minimum adsorption efficiency (35.06%) was also found at pH-4, 25℃, and 3 ml/min. From the adsorption kinetics, the pseudo second order (PSO) was better fitting rate-limiting and controlling adsorption model which explicating that the adsorption was the chemisorption process, and the Langmuir adsorption isotherm was better fitting adsorption isotherm model, and describes the adsorption was a monolayer adsorption process. From the thermodynamics analysis the ΔG was negative, while ΔH, and ΔS were positive, those describe that the adsorption process was spontaneous, endothermic, and the adsorbent has a high affinity for the adsorption of azo-dyes. Generally, graphenecomposite biochar was significant for dyeing-waste water treatment with an efficiency of 99.91% using fixed bed column and easily regenerative adsorbent with regenerative efficiency of (99.897%). Keywords, Water Hyacinth; pyrolysis; graphene containing bio-char; adsorption;