dc.description.abstract |
Food safety is a major global concern, particularly in developing countries. Vegetables
and fruits are vital components of the human food and consuming them regularly is
recognized as a key factor in improving health. However, cont amination of vegetables,
mainly through polluted water irrigation, poses a serious challenge. This study aims to
assess the microbial quality and heavy metal concentration in Ethiopian kale, Swiss
chard, and lettuce samples which were collected from Bahir Dar and Merawi vegetable
gardens. Aerobic plate count, Total coliform, staphylococcus aurous, Escherichia coli
and salmonella of these vegetables were determined by APHA (Plate count, most
probable method) and ISO Methods. The concentration of chromium, cadmium, lead, and
arsenic in these vegetables was determined by using Atomic Absorption
Spectrophotometry. Aerobic plate count bacteria was higher in Ethiopian kale from Peda
(5.82±0.038 log cfu/g) compared to Ethiopian kale from Merawi (4.74±0.520 logcfu/g).
The total coliform result for Swiss chard from Merawi was 6.15±0.029 logcfu/g, while for
lettuce from Peda it was 3.860±0.95 logcfu/g. Ethiopian kale from Peda had a
staphylococcus aureus count of 4.60±0.211 logcfu/g, whereas from Merawi it was
3.86±0.43 logcfu/g. Swiss chard from Gamby and Merawi vegetable gardens were
contaminated with E. coli at values of 2.81±0.567 MPN/g and 2.25±0.43 MPN/g,
respectively. The concentration of Cr was 2.36 mg/kg in Swiss chard and 2.81 mg/kg in
lettuce from the Peda ve getable garden. The concentration Cd was 2.26 mg/kg in Swiss
chard from the Peda vegetable garden, while 2.52 mg/kg of was recorded in lettuce from
the Peda vegetable garden. The study found that the microbial quality of leafy vegetable
samples exceeded the recommended level for indicator bacteria, indicating poor
microbiological quality. Heavy metal analysis revealed that cadmium and lead levels
were exceeded the safe limit, in all sampled vegetables. But almost all vegetables had
lower Chromium concentration which was below the safe limit. Overall, the study
highlighted that contamination of leafy vegetables with heavy metals and microbes in
urban areas, posing a health risk. Notably, salmonella and arsenic were not detected in
the study samples of leafy vegetables.
Key Words: Microbial Quality, Heavy Metals, Vegetables, Contamination |
en_US |