BACKGROUND:-Blood donation is a technique in which blood is collected from a willing and healthy person for the purpose of transfusion to someone else. WHO estimates that it is necessary to donate blood to 2% to 3% of the country's population to meet the most basic blood needs of the country. Blood donation has remained a challenge in developing countries. In Ethiopia, the lack of voluntary blood donors is a major challenge and there is limited information on factors influencing blood donation practices among healthcare providers. Thus, this study aimed to answer the factors influencing practice of blood donation among healthcare providers of public hospitals in Bahirdar city. OBJECTIVE:-The objective of the study is to identify the factors influencing blood donation practice among health care providers of public hospitals at Bahir Dar City. METHODS:-Institution based unmatched case control study was conducted in Bahir Dar city public hospitals from May 01 to May 25 /2022. Total sample size was 491 (123 cases and 368 controls) and then the study subject was selected by using simple random sampling technic and collect data through self-administered questionnaire. Bi variable and Multi variable binary logistic regression analysis were applied to know the association between dependant and independent variables. Finally, the results were presented by charts and tables and report AOR, CI. P-value <0.05 were considered as statistically significant. RESULTS:-Fear of Anemia(AOR:0.02:95%CI 0.007-0.078),lack of opportunity(AOR:0.42: 95%CI 0.22-0.83),Lack of time(AOR:0.03:95%CI 0.005-0.199), profession(AOR:0.15: 95%CI 0.15(0.05-0.42,Aware of free medical checkup(AOR:31.79:95%CI 13.13-76.94),Willingness to donate blood(AOR:5.09:95%CI 2.25-11.50), Blood group type(AOR:5.67:95% CI 1.42-22.68), and Higher work experience(AOR:7.99:95%CI 2.59-24.67)were found to be significantly associated with blood donation practice. CONCLUSION:-The study revealed that fear of Anemia, Lack of opportunity, lack of time, MD by profession, Aware of free medical checkup, willingness to donate blood, blood group type and higher work experience, as influencing factors for the practice of blood donation among healthcare providers of public hospitals. KEY WORDS: Blood donation practice, factors, Bahir Dar LIST OF ABBREVIATIONS