Background: COVID-19 is still a major global threat for which vaccination remains the ultimate solution to protect it; healthcare workers are the first frontiers to fight against the COVID-19 virus, which makes them at higher risk of this disease. Objective: To assess COVID-19 vaccination hesitancy and associated factors among health-care workers in a government hospital in Bahir Dar, 2022. Methods: An institutional-based cross-sectional study design was triangulated with a qualitative study in Bahir Dar from May 22 to June 24, 2022. The quantitative portion of the study used self-administered questionnaires to collect data from 383 health care providers using a simple random sampling technique; for the qualitative portion, 14 purposefully selected health care workers participated in an in-depth interview. SPSS version 20 was used, along with multivariable logistic regressions and Atlas.ti thematic analysis. Result: More than half of healthcare workers (217, or 56.7%) were hesitant to receive the COVID-19 vaccine. Younger age (AOR = 0.53, CI: 0.36, 0.79), Male sex (AOR = 0.56, 95% CI: 0.32, 0.98) and attitude (AOR: 0.31, 95% CI (0.2-0.5) was associated with COVID-19 vaccine hesitancy. Conclusion: COVID-19 vaccine hesitancy among healthcare workers in Bahir Dar, Ethiopia was 56.7%. Male sex, younger age and attitude were associated factors. These results can inform interventions to encourage the uptake of COVID-19 vaccines in healthcare workers. Key words: Attitudes, perceptions, vaccination refusal,