Background: Vaccination is the administration of agent-specific, but safe, antigenic components that in vaccinated individuals can induce protective immunity against the corresponding infectious agent. Vaccines are generally very safe. Measles is one of the most infectious human diseases and can cause serious illness, lifelong complications and death. Prior to the availability of measles vaccine, measles infected over 90% of children before they reached 15 years of age. Objective: To assess dropout and associated factors of measles second dose vaccination in children age of 24-35 months in Hulet Ejuense, East Gojjam zone, Amhara region, Ethiopia. Methods: community based cross sectional study was conducted at Hulet Ejuense woreda from February-May/ 2022. Multistage sampling- technique was used to select the study subjects. A total of 561 participants were selected using systematic random sampling technique. A structured questionnaire was used to collect data. EpiData version 3.1 and SPSS version 23 was used for data entry and analysis respectively. By using descriptive statistics, the frequency distribution of both dependent and independent variables were done. Bivariable logistic regression was done to identify candidate variables. Variables in bivariable with p-value<0.2 was entered into multivariable logistic regression analysis. Finally, significantly associated variables were identified with p-value <0.05. . The goodness-of-fit statistics for the model was done using the hosmer and lemenshow test Results: From the total 552 children, 94 (17.03%) were dropped out MCV2 vaccination in study area. In this study, waiting time for seeking vaccination service longer than 30 minutes (AOR= 2.75, 95%CI: 1.57-4.8), fear of side effect (AOR=2.45, 95% CI: 1.33-4.49), poor knowledge of caregivers (AOR= 8.46, 95% CI: 4.68-15.29) and sickness of child were significantly associated with measles second dose vaccination dropout. Conclusions and recommendation: The finding of this study revealed that MCV2 vaccination dropout rate is high. This findings show that waiting time longer than 30 minutes, fear the side effect and poor knowledge of caregivers for vaccination showed an effect on MCV2 vaccination dropout. Therefore, working to minimize the waiting time for vaccination, provide awareness creation to caregivers on myths of side effect, improving the knowledge of mothers on MCV2 and HEW should have close contact with the defaulter tracing of children who took MCV1 Key words: Measles, MCV2, Dropout, dropout rate, Hulet Ejuense, Ethiopia