Introduction: Integrase strand transfer inhibitors (INSTIs) are recommended for the treatment of HIV infection. There is no consolidated study which assesses DTG viral load suppression rate and adherence in Ethiopia as well as this region. This study would help to promote evidence based treatment practice in chronic HIV care follow up in our locality and country as well. The study aimed to estimate the frequency of individuals with a virologic response up until the 24th weeks after initiating antiretroviral treatment. Methods: Facility based crossectional study was conducted among 351 RVI clients who have been on Dolutegravir based regimen TLD (Tenofovir-Lamivudine-Dolutegravir) in felegehiwot compressive specialized referral hospital within two month period in2021/2022G.c. The study participants were selected using systematic random sampling among clients on TLD regimen of minimum of 6 months. The study was carried out at 24weeks and 48weeks of therapy on TLD regimen during April 2022 to august 2022G.c at felege hiwot compressive specialized referral hospital. The study data were including both before and after TLD regimen initiation from medical chart review. This study used descriptive statistics, binary logistic regression and multivariable binary logistic regression during data analysis method. Variables with P-value less than 0.05 were considered statically significant. Result: In this study, all selected 351 clients charts were reviewed. The mean ages of clients were 41years with standard deviation 11.15 years with [95%CI-40.1-42.5]. Half of, 178(50.7%) were female and 173(49.6%) were male. The proportion of virologic suppression at 24weeks and 48weeks were both 96.9%. Factors, Poor adherence, less than 85% intake rate of medication, [AOR =7.6, (95% CI-3, 18)] and high base line WHO clinical stage, WHO stage III, IV, [AOR=3.2, (95%CI-1.65, 6.3)] were significantly associated with unsuppressed viral load level. Conclusion: Dolutegravir based regimen, TLD regimen, result in significant virologic suppression rate in RVI client. Poor adherence and baseline WHO stage were associated with poor virologic suppression rate. Therefore, designing effective way to improve adherence level and early case identification of RVI clients and enrolled in chronic HIV care is needed. Keywords: DTG, TGSH, Second Line, TLD, ART