Background: Hepatitis B is a potentially life-threatening liver infection caused by the hepatitis B virus (HBV). WHO Report estimated that 40% of HBV infection is a result of occupational exposure, it has been estimated that 14.4% of hospital workers are infected with HBV. Also the prevalence among healthcare professionals in Ethiopia was 7.3–9.0%. The infection is highly contagious, which is recognized as occupational hazard for Health care workers (HCWs), and non-immunized health professionals are at higher risk of acquiring the infection from their work. There is limited information regards on hepatitis B vaccination and factors in the study area. Objective: The aim of this study was to assess magnitude of hepatitis B vaccination status and associated factors among health care workers, working in hospitals of Bahir-Dar city, 2022. Methods: Institution based cross-sectional study was conducted on randomly selected 367 HCWs in hospitals of Bahir Dar city. The samples size was allocated proportionally based on the number of HCWs in the institutions and a simple random sampling technique was used to select study participants. The data was collected by using structured self-administrated questionnaire. The collected Data was entered using Epi-Data version 3.1 and exported to SPSS version 25 for analysis. Descriptive statistics (frequency) and binary logistic regression were used. Independent variables with P-values less than 0.05 were taken as statistically significant Results: - Among 349 health care workers, 197(56.4%)(95%CI: 51.6%–61.7%) had ever received a hepatitis B vaccine. From multi-variable logistic regression analysis work experience less than five years [AOR = 0.15, 95% CI (0.037, 0.628], taking training on infection prevention [AOR = 6.05, 95% CI (3.06, 11.96)], monthly income greater than 5000 ETB [AOR = 3.94, 95%CI (1.64, 9.48)] midwifery professionals [AOR = 4.19, 95% CI (1.32, 13.29)] and being pharmacist [AOR = 3.44, 95% CI (1.09, 10.87)] were significantly associated with hepatitis B vaccination status of HCWs. Conclusion: HBV vaccination status was relatively high compared to other studies conducted in Ethiopia. Taking training on infection prevention, having work experience, having monthly income greater than 5000 and profession were statistically significant predictors for hepatitis B virus vaccination among health care workers. Giving training on infection prevention and free or reasonable cost of the vaccine is recommended to improve hepatitis B vaccination status. Key words: hepatitis B, vaccination status, factors, health care workers, hospitals, Bahir Dar.