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Production and Optimization of Bioethanol from Mixtures of Coffee Husks and Sugarcane Molasses by Saccharomyces cerevisiae

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dc.contributor.author Haimanot, Aragaw Kassie
dc.date.accessioned 2022-11-10T10:52:13Z
dc.date.available 2022-11-10T10:52:13Z
dc.date.issued 2022-08
dc.identifier.uri http://ir.bdu.edu.et/handle/123456789/14334
dc.description.abstract One of the most promising lignocellulosic biomasses for the production of biofuels is coffee husk. Bioethanol production from pure coffee husk is still limited by its high process cost and low hydrolysis and fermentation efficiency. Sugarcane molasses, as a carbohydrate-rich biomass, can provide more reducing sugars for ethanol production. Herein, to reduce high processing costs, molasses was integrated into lignocellulosic ethanol production in batch modes to boost the final ethanol concentration and yield. The production of bioethanol addresses a number of challenges, including the rising cost of conventional fuels, energy security, and climate change. This study aims to reduce agro industrial waste by producing value-added products from it in order to improve Saccharomyces cerevisiaecapacity to produce bioethanol. The conversion of biomass to bioethanol involves pretreatment, hydrolysis, fermentation and distillation. Pretreatment of the biomass was carried out in microwave using acetone as socking agent than dilute by acid hydrolysis (1% H2SO4) the reducing sugar content was measured by Di-nitro salicylic acid method and results maximum of 59.46g/ml reducing sugar. The fermentation process was carried out in static condition at temperature of 25±3℃ using plastic container in anaerobic condition and finally distillation is takes place using rotary evaporator at 85℃. Response Surface Methodology (RSM) a statistical model for the optimization of reducing sugar conditions such as media pH (4-6), hydrolysis time (24-48hr) and mixing ratio of (1:3) was developed. The coffee husk possesses highest cellulose (55.11%) and hemicellulose (25.6 %).The optimum ethanol yield of (58.2) was obtained in a mixing ratio of (1:3), FTIR spectroscopy peaks associated with O-H, C-O, and C-H stretching and vibrations confirmed the presence of ethanol in the product. The result of this study confirms that mixing of coffee husk and cane molasses is a potential feedstock for bio ethanol production. Integrating sugarcane molasses into sequential cellulosic biofuel production could improve the utilization of biomass resources. Key words: Bioethanol, Lingo-cellulose, Hydrolysis, Coffee husks & Sugar molasses en_US
dc.language.iso en_US en_US
dc.subject CHEMICAL AND FOOD ENIGINEERING en_US
dc.title Production and Optimization of Bioethanol from Mixtures of Coffee Husks and Sugarcane Molasses by Saccharomyces cerevisiae en_US
dc.type Thesis en_US


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