dc.description.abstract |
One of the most promising lignocellulosic biomasses for the production of biofuels is coffee
husk. Bioethanol production from pure coffee husk is still limited by its high process cost and
low hydrolysis and fermentation efficiency. Sugarcane molasses, as a carbohydrate-rich biomass,
can provide more reducing sugars for ethanol production. Herein, to reduce high processing
costs, molasses was integrated into lignocellulosic ethanol production in batch modes to boost
the final ethanol concentration and yield. The production of bioethanol addresses a number of
challenges, including the rising cost of conventional fuels, energy security, and climate change.
This study aims to reduce agro industrial waste by producing value-added products from it in
order to improve Saccharomyces cerevisiaecapacity to produce bioethanol. The conversion of
biomass to bioethanol involves pretreatment, hydrolysis, fermentation and distillation.
Pretreatment of the biomass was carried out in microwave using acetone as socking agent than
dilute by acid hydrolysis (1% H2SO4) the reducing sugar content was measured by Di-nitro
salicylic acid method and results maximum of 59.46g/ml reducing sugar. The fermentation
process was carried out in static condition at temperature of 25±3℃ using plastic container in
anaerobic condition and finally distillation is takes place using rotary evaporator at 85℃.
Response Surface Methodology (RSM) a statistical model for the optimization of reducing sugar
conditions such as media pH (4-6), hydrolysis time (24-48hr) and mixing ratio of (1:3) was
developed. The coffee husk possesses highest cellulose (55.11%) and hemicellulose (25.6
%).The optimum ethanol yield of (58.2) was obtained in a mixing ratio of (1:3), FTIR
spectroscopy peaks associated with O-H, C-O, and C-H stretching and vibrations confirmed the
presence of ethanol in the product. The result of this study confirms that mixing of coffee husk
and cane molasses is a potential feedstock for bio ethanol production. Integrating sugarcane
molasses into sequential cellulosic biofuel production could improve the utilization of biomass
resources.
Key words: Bioethanol, Lingo-cellulose, Hydrolysis, Coffee husks & Sugar molasses |
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