Water quality is measured by several parameters, such as the concentration of bacteria levels, the amount of salt, or the amount of material suspended in the water (turbidity). In some bodies of water, the concentration of microscopic algae and quantities of pesticides, herbicides, heavy metals, and other contaminants may also be measured to determine water quality. The main objective of this study was to test the quality of drinking water in Bahir Dar city generated from different sources. Eight sampling sites were selected to represent the source of water quality variations based on purposive sampling method. 8 triplicate water samples (6 from ground water and 2 from springs) were collected from the study areas. The water samples were subjected to the analysis of the physicochemical and bacteriological water quality parameters. The Calcium, Magnesium, Manganese, Iron and Boron of the water sample were determined using Atomic absorption spectrophotometer. Sodium and Potassium levels of the water samples were determined using Flame emission spectrophotometer. Bicarbonates and Sulfates of the water samples were measured by EDTA titration method. The membrane filter technique was utilized for microbiological water quality analysis. The temperature, turbidity, pH, EC, TDS, total hardness, total alkalinity, nitrate, phosphate, sulfate, chlorides, and bicarbonate levels of the analyzed water samples were in the ranges of 22.37-22.83 °C, 2.87- 4.97 NTU, 6.07-7.35, 149- 298.67 μS/cm, 102.17-194.33 mg/l, 54-154.33 mg/l, 67-193.67 mg/l, 14.33-33.67 mg/l, 0.39-33.3 mg/l, 9.33-16.33 mg/l, 1.1-8.73 mg/l, and 85.67-234 mg/l, respectively. Carbonate was not detected at all sampling sites. The Calcium, Magnesium, Manganese, Iron, Boron, sodium and potassium levels were in the range of 8.67-23 mg/l, 13.33-35.67 mg/l, 0.002-0.005 mg/l, 0.001-0.0267 mg/l, 0.01-0.017 mg/l, 3.83-9.87 mg/l, and 1.2-10.13 mg/l, respectively. The average total and fecal coliforms ranged from 0-1294.67 CFU/100 ml and 0-929 CFU/100 ml, respectively. The potassium level of the water samples at GM, GUB, CHR, and AS surpassed the WHO and Ethiopian drinking water quality standards. The pH of the water samples at GUB, CHR, AS, TKR-1, LOM and AR were below the drinking water quality standards. The total and fecal coliform counts of the water samples at GM, GUB, CHR, AS, LOM and AR were found to be above the WHO and Ethiopian drinking water quality standards, 0 CFU/100 ml. The bacterial content of the water samples at GM, LOM and AR were grossly polluted and not in harmony with the reference values set out by WHO and Ethiopian drinking water quality standards. Thus, the water quality of the studied sites was not found in the safe limit of human drinking water. The pollution of these drinking water sources could be associated with the discharge of animal and human feaces, and chemical and fertilizer leachates from agricultural activities.