Background: Hepatitis B infection has been a major public health threat that affects nearly two billion people worldwide with 325 million chronic cases and more than 1.4 million deaths every year. Approximately two million health care workers globally each year are at risk of becoming infected with hepatitis B virus through their routine duties in their workplaces, and very few health care workers are vaccinated. Universal vaccination of health-care workers is an effective strategy to protect from hepatitis B infection. Objective: - To determine hepatitis B vaccination status and its associated factors among health care workers working in primary hospitals and health posts of Central Gondar zone woredas. Method: - An institution-based comparative cross-sectional study was conducted from March 15 to April 15, 2020. A multi-stage sampling technique were used. A self-administered questionnaire were used to collect the data. Data were entered into Epi data version 3.1 and analyzed with a statistical package for social science version 23. All variables at p-value < 0.25 in the bi-variable analysis were considered for further multi-variable binary logistic regression analysis to detect the significance of vaccination status. Results: The overall prevalence of hepatitis B vaccination among health care workers was 43.5% (95%CI; 40.2-46.9). The prevalence of vaccination was 56.6% (95%CI; 51.2-62.0) in primary hospitals and 34.6% (95%CI; 30.2-39.1) in health posts. The odds of vaccination were higher among health care workers working in primary hospitals [AOR=2.681(95% CI: 1.3-5.4)], being married AOR=2.468(95%CI: 1.5-4.1)], degree and diploma holders [AOR=2.934(95%CI: 1.4-6.2)], and [AOR =1.740(95%CI: 1.1-2.8)] respectively, taking infection prevention training [AOR=5.738(95%CI:3.8-8.7)], needle-stick injuries [AOR=1.721(95%CI: 1.1.2-7)], and pre-vaccination screening [AOR=5.123(95%CI: 2.9-8.9)]. Conclusion: The finding of this study generally shows a low rate of hepatitis B virus vaccine coverage among health care workers working in Central Gondar zone primary hospitals and health posts. Therefore, there is a need for well-planned program to vaccinate health care workers and offer pre-vaccination screening test before vaccination to improve the vaccination coverage of health care workers in order to protect them from acquiring hepatitis B infection. Keywords: - Healthcare workers, Hepatitis B virus, Central Gondar zone