Background: Inter-professional collaboration is defined as working together for the same goal, working with agreement, having a good communication and exchange of ideas, listening to each other, meeting the needs of other professionals, being available to each other and achieving high quality of patient care. In health care, where the majority of actions are performed as a team, inter-professional collaboration is critical to improve the quality service provided. Many health systems and health professionals around the world are disconnected and overwhelmed to meet unmet health demands. Objective: To assess inter-professional collaboration of health professionals during health care provision and associated factors at hospitals in Bahir dar city, Northwest, Ethiopia, 2022 Methods: A Cross-sectional study with both quantitative and qualitative method was conducted among 380 health professionals at hospitals in Bahir dar city. The study participants were selected using a stratified sampling technique and stratified by profession, systematic random sampling was employed to select each study participants. The quantitative data were collected using a structured self-administered questionnaire and the qualitative data was collected by FGD guided interview. Purposive sampling technique was used for qualitative study. SPSS version 23 and ATLAS ti7 data analysis software were used for quantitative and qualitative data respectively. The level of significance is set at a p< 0.05 Result: A total of 380 respondents with a 98.68% response rate were included. Around two third, 353(67.5%) respondents had favorable IPC. Factors higher institution learning background of respondents (AOR=2.32, CI: 1.37-3.94), culture of teamwork the organization (AOR=2.21, CI: 1.27-3.84), physical environment and rule and regulation of the organization (AOR=2.47, CI: 1.38-4.40) were found statistical significance. Conclusion: This study shows, most of health professionals had favorable IPC during health care provision. It was relatively good compared other related studies. Keywords: Inter-professional collaboration, health care provision, Bahir dar city