Background: Despite ranges of anti-malaria interventions worldwide, it lasts one of the top public health problems causing morbidity, mortality and high cost for care. It has varying magnitude across countries and has highest burden in developing countries including Ethiopia. Having updated evidence on the status of malaria distribution, trends and its associated factor is important to take evidence based anti-malaria interventions. Objective: The objective of this study was to assess the trend and associated factors of malaria infection in Bahir Dar City administration, Northwest Ethiopia. Method: A retrospective study was conducted on seven years’ (2014-2020) zonal data. To determine factors associated with malaria infection, malaria records of 2020 were collected from randomly selected laboratory logbook of health facilities. Data were exported from excel sheet to SPSS version 20 software for cleaning and analysis. Descriptive statics such as overall malaria prevalence, and trend of malaria by age, sex, year and seasons were computed. Multivariable logistic regression analysis was used to identify factors associated with malaria infection. The strength of association was described using odds ratio at 95% of CI and p-value less than 0.05. Result: A total of 436,591 patients were tested for malaria at Bahir Dar city administration from 2014 to 2020. And 44,855 were reported as malaria cases and 43,415 (97%) of them were reported as confirmed malaria cases with the rest 1440 (3%) were clinical cases. The overall malaria infection in the present study showed a fluctuating trend with a decreasing order from 2014 to 2018 and increasing sharply for the last two years 2019 to 2020. Plasmodium falciparum and plasmodium vivax accounted for 30044(69 %) and 12648(29%) and the rest clinical, respectively. Age, area of residence and season of the year were statically significant with malaria infection at P-value < 0.05. Conclusion and recommendations: Malaria is still a public health burden in the study area which characterized by progressive decline for 5 years and sudden increase in the last 2 years Therefore it needs further investigation like community- based assessment to determine possible contributing factors and interventions according to malaria prevention and control strategies. Key Words: Malaria, trend, Prevalence, seasonal distribution, Bahir Dar, Ethiopia