Background: Premature rupture of membranes (PROM) is a rupture of the membranes one hour before the onset of labor. PROM occurs in about 10 percent of all pregnancies. It estimated to cause of 50% preterm births with a fourfold increased risk of fetal mortality. and 80% maternal infection worled wide. and with a relatively higher prevalence in Africa. Premature rupture of membrane (PROM) is linked to significant maternal prenatal mortalities and morbidity. In Ethiopia, Little is known regarding the determinant of PROM in Ethiopia and previous studies on risk factors of PROM were utilized secondary data from health institution which are subjected to miss important variable like frequency of ANC visits.Then this study will narrow the evidence gap, and it may show findings for responsible bodies of health care system, and to prevent determinants of PROM. Objective: To identify the determinants of Premature rupture of membrane among laboring women in public hospitals at Bahir Dar City, Northwest Ethiopia Methods: An institutional based casecontrol study design was implemented among 284 participants (213 controls, 71 cases).Women with PROM were recruit consequently as cases and women without PROM admitted following the cases was selected using systematic random sampling technique as controls. Data was collected by using interviewer administered structured questionnaire and checklist. Data were entered to EPI data version 4.6 and export to SPSS version 23 for analysis. Binary logistic regression model was used to see the association between independent and dependent variable. P-value < 0.2 in bi-variable analysis were candidates for multivariable analysis and P-value <0.05 was used to declare as statistically significant. The odds ratio (OR) with 95% confidence interval (CI) was used to measure the strength of association. Results: Multivariable analysis showed that history of urinary tract infection [AOR 7.3(CI: 2.736,19.501)], Anemia [AOR3.213(CI:1.428,7.230)], History of premature rupture of membranes [AOR10.462(CI:2.449,44.693)], and less than four ANC visits [AOR4.554 (CI: 2.010,,10.320)] were positively associated with premature rupture of membranes. Conclusions: Urinary tract infection, previous history of PROM ,Anemia and Ante natal care visits are diterminat factors for Premature rapture of memberane. Strengthen focused ANC follow up, diagnose, screening and early initations of treatment of urinary tract infections, anemia and provide iron folate with more than 90 tablets during pregnancy will reduce PROM. Keywords: Premature rupture of membrane, Determinants, Case–control study Ethiopia