Background; Acute respiratory infection in children is infection of the respiratory system and characterized by cough accompanied by short, rapid breathing that is chest-related, and/or difficult breathing that is chest-related . Acute respiratory infection is a major cause of morbidity and mortality in young children worldwide. In Ethiopia ARI related mortality and morbidity still high. Because of huge difference in prevalence and risk factors of ARI in regional states of Ethiopia, identifying associated factors of ARI for Amhara region is essential to take appropriate intervention. Furthermore there is limited information regarding associated factors of ARI in under-five children at Amhara regional level and most of studies in Amhara region were conducted at Woreda, Town and health facility level and they used small sample size. Therefore, this study was aimed at identifying prevalence and associated factors of ARI in Amhara Region by using regionally representative data from EDHS 2016. Objective: To determine the prevalence and associated factors of acute respiratory infection among under-five children in Amhara Region, Ethiopia, 2020. Methods: Community-based cross-sectional study was carried out involving 1967 children under the age of 5 years. Information pertinent for this study was extracted from the main data set and a working data set was prepared. Analysis of the data was performed using STATA 14. Multilevel mixed effect binary logistic regression was applied to identify associated factors. Results: The prevalence rate of acute respiratory infection in Amhara region was 7.9% (95% CI=0.062, 0.097). The odds of ARI in children of age between 6-11 months were 4 times as compared to those age between 0-6 months (AOR= 3.33, 95% CI: 1.04, 10.71), The odds of ARI in children who had diarrhea were 5 times higher as compared to had no diarrhea (AOR= 4.72, 95% CI: 2.48, 8.96), The odds of ARI among children who had fathers with primary level of education were reduced by 54% as compared to had no education (AOR=0.44, CI: 0.20, 0.99), Children of mother’s age group 15-19 years old have increased the odds of ARI by 4 times as compared to age group 35-49 years(AOR= 4.22, CI: 1.05, 16.92) and the odds of ARI among children who distance to the health facility were a big problem were increased by 82% as compared to their counterpart (AOR: 1.82; 95% CI: 1.02, 3.24) were associated factors of under-five acute respiratory infection. Conclusion: In this study, the prevalence rate of ARI was slightly higher than national prevalence. Child age, children had diarrhea, Father Education level, distance to health facility and mother’s age were associated with acute respiratory infection. Governments should continue to strengthen provision of education and provide information on ARI for all community. Key words: ARI, Associated factors, Ethiopia.