Background: Cervical cancer is a malignant neoplasm from cells originating in the cervix. Cervical cancer screening is one of secondary prevention mechanism. According to the WHO report in developing countries screening coverage is still low ranging from 2.0% to 20.2% in urban areas and 0.4% to 14.0% in rural areas. Low screening coverage in our country cause most patients to present to hospital with advanced disease. Objective: To assess cervical cancer screening practice and associated factors among female health service providers in Bahir Dar city administration between September to October 2018. Methods: A cross-sectional study was conducted among 388 participants. Stratified and simple random sampling proportionate to size allocation technique was used. Data were collected using a self-administered structured questionnaire. Data were entered in to EPI-INFO version 3.5.4 and analyzed using SPSS version 23 software. By using descriptive statistic proportion of screened for cervical cancer was determined. Binary logistic regression analysis with a cutoff p- value ≤ 0.2 was used for variables to be entered into multivariable logistic regression analysis. Adjusted odds ratios with 95% CI were used to identify independent predictors of cervical cancer screening practice. Independent variables that had a p-value less than 0.05 in multivariable logistic regression analysis were taken as statistically significant factors for cervical cancer screening practice. Results: The mean age (+SD) of the study participants was 34.48 (+4.15 year) with the minimum and maximum age range of 27 and 58 years respectively, 223 (57.5%) were degree holder, 339 (87.4%) were married and 229 (59%) were nurses. Practice of cervical cancer screening was found to be 17.3 % (95% CI: 13% to 21%). Age ≥35 (AOR:2.89;95% CI:1.37-,6.10), working in screening center (AOR:0.26;95% CI:0.14-0.51), age at first sexual contact<18 year (AOR:0.41;95% CI:0.20-0.87) and use of contraceptive (AOR: 0.37;95% CI:0.18-0.76) were found to be significant factors for cervical cancer screening at p-value less than 0.05. Conclusion: Cervical cancer screening practice among female health service providers was found low and significantly associated with age, working screening center, use of contraceptive and age at first sexual contact before18 years. Keywords: cervical, cancer, screening, practice, female health service providers, Bahir Dar city, Ethiopia