Back ground: - Intestinal parasitic infection is one of the parasitic infections affecting people living in prison. Helminthes and intestinal protozoan infections are the most common parasitic infection that may cause serious life-threatening diseases in inmates living in developing countries. Objective: - To determine the prevalence of intestinal parasitic infections and associated factors among Awi Zone prisons: Amhara region, Ethiopia, 2020. Methods: - Institutional based cross-sectional study design was conducted from May, 2020 to January, 2021 with a total of 422 inmates in Awi Zone prisons. Proportional sample size allocation was used to determine the required sample size from each prison. Data were collected by pretested structured questionnaire and stool samples were collected from inmates by laboratory professionals. The data quality was maintained during data collection and before analysis by checking for its completeness and recording. The data were entered to Epi data version 4.6 and exported to SPSS version 20 for data cleaning and analysis. Bivariate and multivariable analysis, variables with p<0.05, were taken as statistically significant and independently associated with intestinal parasitic infection. Result: - Among 422 respondents, 413 prisoners gave complete responses with response rate of 98%. The overall prevalence of intestinal parasitic infection was 37.8 %,( 95% CI: 33.2%, 42.4%). Moreover, the study identified that untrimmed finger nail (AOR= 2.761, 95% CI:1.616, 4.717), not washing hands before meal (AOR= 3.353, 95% CI:1.69,6.62), not taking anti parasite drugs (AOR= 2.498,95% CI:1.41, 4.40), poor knowledge about intestinal parasitic infection (AOR= 2.156,95% CI:1.30,3.56) and sleeping in group/together (AOR= 2.397, 95% CI:1.44,3.97) were significantly associated with the prevalence of intestinal parasitic infection among prisoners. Conclusions: - Intestinal parasitic infection is a major health problem among prisoners in Awi zone prisons. The untrimmed finger nail, not washing hands within a day, hand washing once within a day, and sleeping in group/together were associated with intestinal parasitic infection. Therefore, deworming complemented with preventive measures such as provision of health education and improvement of sanitation and hygiene behavior of prisoners must be done.