Background: Despite the high economic and mortality burden of chronic kidney disease, studies on survival and predictors of mortality among patients on hemodialysis in Ethiopia especially in Amhara region are scarce. Which is important to identify some modifiable risk factors for early mortality to improve the patient's prognosis. So, this study was done to fill the identified gaps. Objectives: To assess survival and predictors of mortality among chronic kidney disease patients on hemodialysis in Amhara regional state, Ethiopia, 2020/2021 Method: Institution-based retrospective follow-up study was conducted in Felege Hiwot, Gondar, and Gambi hospitals from March 5 to April 5. A total of 436 medical records were selected using a simple random sampling technique. Data were collected using a data extraction tool then entered into Epi Data version 3.1 and exported to STATA™ version 14.1 for data cleaning and statistical analysis. Data were described using text, graph, table, median, interquartile range, frequency, and percent. A life table was used to estimate probabilities of survival at different time intervals. Predictors on the bivariable cox regression with a P value less than 0.25 were included in the multivariable Cox regression and a P-value less than 0.05 with a 95% confidence interval was considered as a significant predictor of mortality. Result: Out of the 436 patients 153 (35.1%) had died. The median survival time was 345 days with a mortality rate of 1.89 per 1000 person-days (95%CI (1.62, 2.22)). Patients live in rural residence (AHR=1.48, 95%CI (1.04, 2.12)), patients whose cause of chronic kidney disease was hypertension (AHR=1.49, 95%CI (1.01, 2.23)) and human immune virus (AHR=2.22, 95%CI (1.41, 3.51)), and patients who use a central venous catheter (AHR=3.15, 95%CI (2.08, 4.77)) had increased risk of death while staying four hours on hemodialysis (AHR=0.43, 95%CI (0.23, 0.80)) decreases the risk of death among chronic kidney disease patients on hemodialysis. Conclusion and recommendation: The overall survival rate and median survival time of chronic kidney disease patients on hemodialysis were low in Amhara region. Hence strategy should be formulated to prolong the survival time of patients by promoting early screening and diagnosis of chronic kidney disease in Amhara region. Keywords: Survival, chronic kidney disease, Hemodialysis, Amhara region