Background; Health profile is a system of collecting and summarizing health and health related events, demographic, socio-economic, political and cultural aspect of a particular district. It is used for planning, implementation and evaluation of public health programs. Therefore, the aim to determine the magnitude of disease and health status of the population in the district. Objective; To assess and describe the social, economic, geographical, health and health related status and identify problems for priority setting in Dejen district, East Gojjam Zone, Amhara region, Ethiopia. Methods: Descriptive cross-sectional study design was used to assess Health and health related indicators in the district. The data were collected from March 22 to April 12/2018 in Dejen district. The data necessary for Health profile description and were collected from Dejen district Health office, Education office, Culture and Tourism, Town Municipality, Agricultural office, Administrative office, Water and Energy office and Other Sectors reviewing records and using check list for face to face interview of officers. Finally, the data were compiled and analyzed manually and using Microsoft excel sheet 2007. Results: The district population is projected to a total of 121844, that of male population were 58729 (48.2%) about 104709 (86%) population reside in rural areas and agriculture dependent. The health service coverage of the district was 100% which based an estimate of the standard of Health facility. Safe drinking water supply coverage was 78% in rural and 86% in urban communities. About 74.1% of health posts have not water access in their compound. No electric city power supply for 84.7% of Health posts. Delivery attending by skilled professionals, post-natal care and family planning, have coverage of 86.4%,81.9%,86% respectively. the district latrines utilization 68% and coverage was 89.2% the health sector budget allocation of the district was 8.2% the leading cause of disease for outpatient visits in adult were Acute febrile illness and under five pediatric morbidities were Diarrhea (nonblood). Conclusion: The district health profile showed that majority health service indicators ANC4, delivery attended by skilled personnel, postnatal care was under target achievement and most of the health posts have not water and electric access. TB screening and case detection was low. The major health problem disease was Acute febrile illness and Diarrhea (nonblood). Recommendations: Every concerned body should work in collaboration to improve each achievement. community TB screening and case detection should be improved. Prevention and control diarrhea should be given special attention by the woreda health office. Health facilities in the woreda especially Hp's shall be provided with sustainable water & Electric power supply. HIV Screening program should be strengthened. The district administrates should allocate sufficient budget.