Background: Anthrax is a zoonotic disease and it is endemic in Ethiopia. Anthrax preventive measures are the key activities to control the anthrax disease. Several outbreaks of anthrax have occurred in humans in Ethiopia in recent times. However, there is a paucity of information on the anthrax prevention among farmers of Farta district. Objective: To assess the level of anthrax preventive practice and its associated factors among farmers in Farta District. south Gondar zone, Amhara region, North West Ethiopia, 2020. Method: A community-based cross-sectional study design was conducted among Farta District farmers from June 7 to 21/2020. A multi-stage sampling technique with systematic random sampling was used to select 1338 study participants and data were collected via face to face interview using standard questionnaires. The data were cleaned, coded and entered into the Epi-data version 3.1 and then exported to SPSS version 23 for analysis. Missing data was checked by observing frequency results. Both descriptive and binary logistic regressions were employed. A binary logistic regression model was used to identify Factors of the outcome variable. Variables significantly associated with the outcome variable during bi-variable binary logistic regression at p-value <0.2 were included in the multiple binary logistic regressions. Those independent variables with P-values less than 0.05 were taken as statistically significant factors of anthrax preventive practice. The goodness of fit model was checked by Hosmer Lemeshow statistic and a p-value greater than 0.05 was considered as a fit model. Results: The level of farmers who had anthrax preventive practice was 35.8% [95% CI (33%, 38%)]. Being female (AOR:2.96;95%CI:2.18, 4.02), able to read and write (AOR:1.48, 95%CI:1.08, 2.03), time spent to arrive at a nearby veterinary clinic (AOR: 1.35;95%CI:1.01, 1.79), heard about anthrax (AOR:4.54; 95%CI:2.96, 6.95), and had a favorable attitude (AOR:3.70; 95%CI:2.77, 4.94) were significantly associated with anthrax preventive practice. Conclusions: The level of having anthrax preventive practice was 35.8%. Sex, educational status, time spent to arrive at a nearby veterinary clinic, heard about anthrax, and attitude were significantly associated with anthrax preventive practice. Providing health information, creating favorable attitude and availability of a nearby veterinary clinic are recommended to improve anthrax preventive practice in the study area. Keywords: Anthrax, prevention practice, zoonotic disease, Ethiopia