The assessment of macroinvertebrate community of the Enfranz stream, located northwest of Bahir Dar city in the southern part of Lake Tana, was studied in wet and dry seasons from August 2010 to May 2011. This stream feeds Lake Tana and its total catchment area is 198 krrr'. Four sampling sites were selected along the stream. Physicochemical parameters, temperature, dissolved oxygen, conductivity and pH, were measured. Macroinvertebrates were collected using standard dip net based on the Rapid Bio­ Assessment Protocols for use in streams and . wadeable rivers. Most physicochemical parameters varied significantly (P < 0.05) among sampling sites. A total of 15286 macroinvertebrate individuals, belonging to 30 families were identified. Macroinvertebrate metrics for stream water quality assessment were calculated: total Taxon Richness (TR), % Dominant Taxa (DT), Shannon Wiener diversity index (ff), Pielou Evenness Index (J'), Percent Ephemeroptera (%Eph), Percent Trichoptera (%Tri ch), Hilsenhoff Family-Level Biotic Index (HFBI) and Percent Dipterans (%Dip). Results of ANOVA showed that macroinvertebrate metrics differed significantly among sampling sites and seasons (P < 0.05). Pearson's correlation coefficients showed that, physicochemical parameters were significantly correlated with macroinvertebrate metrics. It was found that human activities such as agriculture and grazing changed the bank of stream by reducing the overall water quality and macroinvertebrates, it is recommended to create forested buffer zone that promotes the broader diversity of habitats and protect the stream from point-source and non-point-source pollution. Efforts to assess and monitor biological conditions in this stream should include habitat quality assessment and comprehensive analysis of water quality parameters.