Cross sectional study was conducted in dairy cows from randomly selected households of Machakel District. A total of 375 lactating dairy cows were selected using systematic random sampling technique.CMT was used to screen subclinical mastitis. A Structured questionnaire pre- tested containing close-ended questions were used to collect data regarding the various potential risk factors of mastitis. Binary logistic regression model was used to determine the association between mastitis and its risk factors. Variables with P-value less than 0.25 at individual level (bivariate/univariate) analysis at 95% confidence interval were exported to the multivariate analysis. The existence of association was declared with 95% confidence interval at p value <0.05. A total of 375 lactating dairy cows were included in the study. The mean age of cows was 2.9 ±2.35 years and majorities (258, 68.8%) of them were ≤4 years of age. Of the total 375 cows examined during the study period, 146 (39%) of the cows were positive for mastitis. Out of these, 9% (33/375) and 30 % (113/375) showed clinical and subclinical mastitis, respectively. The quarter level prevalence was found to be 16.1% (242/1500); from which, 12.2 % (183/1441) and 3.9% (59/1500) were found to be of subclinical form and blind teat, respectively. Type of breed(Cross compared to local) [AOR= 2.1, 95%CI =(1.33, 3.32)], stage of lactation (>6 months compared to ≤4 months) [AOR=2.46, 95% CI=[1..35, 4.48], type of housing (Shared barn compared to Separate barn) [AOR=1.94, 95% CI=(1.11, 3.38)], and the practice of washing cow’s udder (Not washed compared to Washed) [AOR=1.65, 95% CI=[1.06, 2.56] were significantly associated with mastitis The prevalence of mastitis has to be reduced to a lowest level possible. Though the finding from this research was somewhat consistent with some and lower than the other previously conducted research findings globally and in Ethiopia as well, vii the prevalence of mastitis dairy cows of Machakel district is still a threat to milk production and public health