Challenges of land use type and soil depth have serious effects on soil properties. Thus, assessing land use types in soil properties are necessary for agro-ecosystem transformation. This study was conducted in Shemit watershed Northwest Ethiopia, with the objective of investigating the effects of land use type on soil properties. Four land use types: natural forest, cultivated land, grazing land and plantation forest were selected to determine the effect of land use types on soil properties. A total of 24 disturbed composite and 24 undisturbed core soil samples were collected from the four land use types at two soil depth in triplicates. The soil samples were prepared and analyzed in the laboratory following the standard procedures. The result showed that cultivated land and settlement increased by 3.65% and 6.33%, respectively while, the forest and grazing lands were declinead by 2.76% and 7.23%, respectively from 1989 to2019. Sand, clay, bulk density, pH, OM, total N, available phosphorus, exchangeable bases (Ca, Mg, K, Na), CEC and PBS were significantly (p < 0.05) affected by land use type. In contrast, silt and C: N ratio were not significantly (p > 0.05) affected by land use. Considering soil depth, only sand, OM, exchangeable Na and PBS were significantly (p < 0.05) varied. The highest mean pH (6.34), SOM (5.32%), TN (0.28%), Av.P (6.24 mg/kg), Ca (14.13 cmol c ), K (1.11 cmol c kg -1 ) and CEC (28.77 cmol c kg -1 ) were recorded under natural forest and the lowest values except for total N, OM, Av.P and CEC were found under plantation forest. The mean value of clay, BD, pH, exchangeable bases and PBS were increased down a depth, whereas, sand, silt, TN, OM and CEC were decreased with increase soil depth. A change from natural forest to other land use types caused a significant decline in soil fertility parameters. Therefore, Shemit watershed needs immediate intervention and implementation of sustainable land management to protect the remnant natural forests and to improve agricultural productivity. kg -1