Beekeeping is one of the agricultural activities in the rural communities and appears as ancient history of Ethiopia. The study was designed to characterize beekeeping production system and performance evaluation of different hive types in different agro-ecologies of Dawunt district, North Wollo zone, Amhara. For this study six kebeles namely, Sholla, Yabtie, Ayda, Sheglla, Dacha and Yesay-Yedba were selected from three agro-ecologies (lowland, midland and highland). A total of 126 beekeepers with different proportion from each kebele were selected randomly. Beekeepers were interviewed using pre-tested semi-structured questionnaire and the data was analyzed using Statistical Package for the Social Sciences (SPSS) version 23. The results of the household survey indicated that the majority of the hives owned by the beekeepers were traditional (54.5%), followed by movable frame (39.4%) and transitional (6.1%) beehives. The sampled beekeepers harvest an average of honey yield 7.56±0.981, 9.06±1.571 and 16.99±2.949kg/hive/year in traditional, transitional and movable frame hives, respectively. Through measuring, the sample beekeepers harvest an average of honey from traditional 10.10±2.183, 13.70±2.869 and 9.20±3.259 kg/hive/year in the lowland, midland and highland respectively, from transitional 14.40±3.718, 18.70±2.751 and 13.00±3.197 kg/hive/year in the lowland, midland and highland respectively and from movable frame hive 17.20±3.360, 21.20±3.084 and 15.70±2.359 kg/hive/year in the lowland, midland and highland respectively with the overall mean of honey yield 11.00±3.353, 15.37±3.987 and 18.03±3.709 kg/hive/year in traditional, transitional and movable frame respectively. It is concluded that, the measured honey yield was greater than that of the honey yield of the survey result and