Team handball is a complex sport game, which requires maximum intensity efforts in a short period of time, where players jump, run, and throw the ball at a high velocity, followed by low intensity or rest moments. The purpose of this study was to investigate the effect of resistance training on shooting accuracy and some selected physical fitness variables. The study employed Experimental research design. 24 male handball players of Woreta city with the age of (EG=15.50, CG =15.4167) were randomly assigned into 2 equal groups. Both the experimental group (EG, n = 12) and control group (CG, n = 12) participated in the regular handball training, but only EG performed additional resistance training for 12 weeks, with 3 sessions per week, each enduring 50 to 60 minutes. Subjects were measured on shooting performance, vertical jump, long jump with one leg take off, agility and 30m sprint time on two occasions first before administration of resistance training as pre-test and after twelve weeks of the training as post-test. The data collected from the study subject was analyzed using SPSS version 23 software by paired t-test and independent sampling test with level of significant 0.05. The results showed that resistance training significantly improved shooting accuracy, explosive power and agility performance in EG at (p<0.05). Similarly, sprint time was significantly reduced (p<0.05). But no significant differences were found in all of the variables in CG (p>0.05). Based on this finding, it can be concluded that twelve weeks resistance training has positive effect on improvement of shooting accuracy and physical fitness variables of handball players. These data recommend that 12-week resistance training, when included into a regular training session, can provide greater physical fitness variables improvements for male handball players Keywords: Resistance Training, shooting accuracy, Explosive Power, Speed, Agility.