Variability in crop response and nutrient use efficiency to nutrient supply in maize is common in the study area due to unbalanced fertilizer application due to the absence of site specific and crop demand based fertilizer recommendation. Balanced fertilization based on soil test and crop demand is imperative for efficient nutrient management and has a considerable role for enhancing maize yield. Thus, a field experiment was conducted with an objective to identify the most yield limiting nutrient (s) in Nitisols of Omo Nada District Southwestern Ethiopia during 2019/20 cropping season through nutrient omission technique. The experiment was laid out in RCBD with ten treatments replicated four times across four farmers’ fields. The treatments were (Control, NP, PKS (-N), NKS (-P), NPS (-K), NPK (-S), NPKS, NPKSZn (-B), NPKSB (-Zn) and NPKSZnB. Soil sample was taken at a depth of 0-20 cm before application of treatments. The results of soil were moderately acidic, sandy clay loam texture, low in total N, available P and medium in K, S, Zn, B, OC, OM and CEC. Maize yield and yield component datum were subjected to ANOVA using SAS 9.3 software. LSD test was used to separate means at 5% level of significant (P < 0.05). Maize grain yield, Biomass yield, Nutrient concentration, uptake and agronomic efficiency were highly affected by nutrient omission. The highest grain yield of maize (8702.6kg ha-1) and biomass (20.13 t/ha) were obtained from application of 120 N + 40 P + 40 K + 20 S + 5 Zn kg ha-1. The lowest grain yield (2028.5 kg ha-1 and 2793.5 kg ha-1) and biomass yield (5.55 t ha-1 and 7.23 t ha-1) were recorded from control and N-omitted plots, respectively. Yield reduction were ranked as omission of N > P > K > S > Zn indicating N was the most yield limiting nutrient, followed by P and K. Compared with NP, the NPKSZn treatment increased maize yield by 29.79 %. The maximum nutrient uptake of N, P, K, S, Zn and B were obtained from plots treated with NPKSZn. The highest agronomic efficiency of N, P, K, S and Zn were also recorded from plots treated with NPKSZn. Moreover, this treatment generated the highest net benefit of 80,364 ETB ha-1with acceptable MRR (486.35%). Therefore, integrated use of major macronutrients (NPKS) and Zn was recommended to increases maize production in the study area. Keywords: Maize, Nitrogen, Phosphorus, Potassium, Sulfur, Zinc, Boron, Limiting nutrients