A cross-sectional study was conducted from November 2019 to April 2020 in Bahir Dar city to investigate the occurrence of Salmonella from poultry farms and in-contact humans and to determine their antimicrobial resistance profiles. A total of 288 samples from poultry cloacal swabs, environmental samples (feed, drinking water, boot swabs) and in-contact humans were collected and processed for Salmonella isolation by using conventional culture methods and then further confirmed by genus specific polymerase chain reaction. Isolated Salmonella species were tested for antibiotic susceptibility to 11 antimicrobials using Kirby–Bauer disk diffusion assay. Questionnaire survey and observations were made to observe the associated determinant factors. Four (18.2%) of the farms and 4 (1.5%) of the samples from poultry farms and 1 (6.7%) of stool sample of humans in-contact with poultry were found to be positive for Salmonella. Poultry farms with large flock size were found significantly associated with the occurrence of Salmonella (p=0.044). Isolation rate of Salmonella between sample types were statistically significant (p=0.031). All Salmonella isolates were susceptible to ciprofloxacin and gentamycin; however, all of them were resistant to tetracycline and they developed multiple drug resistance. The hygiene of the poultry houses were substandard and poultry farms that practice proper use of foot bath were 0.042 times less likely to be contaminated with Salmonella as compared to counterparts (p= 0.021). Therefore, improved management practices and rational antimicrobial usage should be employed and also detailed epidemiological and molecular studies should be conducted to establish sources of acquisition of resistant Salmonella strains. Keywords: Antimicrobial susceptibility; Bahir Dar city; Human; Poultry, Salmonella