dc.description.abstract |
The study was conducted to assess the extent of genetic variability and to determine the
association between quality traits and SNP markers in Ethiopian durum wheat genotypes. Field
experiment was conducted at Debre Elias and Debre Tabor, and One hundred durum wheat
genotypes were evaluated in partially balanced lattice design, 10 by 10 arrangements with two
replications. The analysis of variance revealed highly significant variation (P≤0.01) among the
tested genotypes. GCV and PCV values ranged from 3.71% (ash content) to 46.12% (RDSM)
and 5.78 (ash content) to 60.1% (RDSM), respectively. H 2 and GAM ranged from 6.6% (NET)
and 81% (SL) and 1.49% (ash content) and 81% (RDSM), respectively. Grain yield showed
highly significant (P<0.01) genotypic and phenotypic correlation with biomass (0.77, 0.79),
respectively. The cluster analysis grouped the genotypes into 8 and 12 major groups consisting
of one to 86 genotypes at Debre Tabor and Debre Elias, respectively. The first six principal
components explained 78.1% and 78.88% of the total variation at Debre Tabor and Debre Elias,
respectively. The same genotypes were also evaluated for genome wide association study
(GWAS) using 4809 single nucleotide polymorphisms (SNPs) with the general linear model.
Highly significant differences among genotypes were detected for all traits. Totally, 75 MTAs for
agronomic, quality and disease traits were identified on all chromosomes. A total of 75 highly
significant (P < 0.001) MTAs were identified with R2 ranged from 17.03 to 32.86%. The study
revealed association of molecular markers with agronomic and quality traits. However, the field
study was conducted for one season at two locations, which needs to testing subsequent breeding
trials at different locations to develop high yielding variety and the amenable of GWAS.
Keywords: SNP, QTL, GWAS, Durum wheat, Morphological marker |
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